Cancer Research

5.  Title

Chemopreventive Activity of Maharishi Arnrit Kalash [MAK-4 and MAK-5] and Related Agents in Rat Tracheal Epithelial and Human Tumor Cells

Publication

Proceedings of the American Association for Cancer Research, Vol. 32, p. 128, 1991 (Abstract).

Authors

J.T. Arnold,* B.P. Wilkinson,* E.A. Korytynski,* and V.E. Steele.**

Conducted at

* ManTech Environmental Technology, Inc., RTP, NC 27709

**National Cancer Institute, Bethesda, MD 20892

Summary

The rat tracheal epithelial (RTE) cell focus inhibition assay and the A427 human tumor cell anchorage independent assay were used to evaluate the chemopreventive potential of two medicinal Ayurvedic herbal mixtures, MAK-4 and MAK-3. A 1:1 aqueous:ethanol extract of MAK-4 inhibited benzo[a]pyrene-induced morphological transformation in the RTE assay by 27% and inhibited growth of the A427 tumor cells in soft agar by 31%. An aqueous extract of MAK-3 inhibited transformation in RTE cultures exposed to benzo[a]pyrene by 53%. These results suggest that MAK-4 and MAK-5 inhibit processes of neoplastic transformation. Compounds tested in these assays that are similar to the ingredients in MAK-4 and MAK-5 also show chemopreventive activity. These include curcumin, ferulic acid, catechin, bioflavonoids, retinoic acid, ascorbyl palmitate, and glycyrrhetinic acid.

6.  Title

Antioxidant Adjuvant Therapy Using Natural Herbal Mixtures [MAK-4 and MAK-3] During Intensive Chemotherapy: Reduction in Toxicity. A Prospective Study of 62 Patients

Publication

Rao, R.S., Deo, M.G., and Sanghvi, L.D. (eds). Proceedings of the XVI International Cancer Congress. Bologna, Italy: Monduzzi Editore, 1994: pp. 3099-3102.

Authors

N.C. Misra,* H.M. Sharma,** A. Chaturvedi,* Ramakant,* S. Srivastav,* V. Devi,* P. Kakkar,t Vishwanathan.t S.M. Natu,* andJ. Bogra*

Conducted at

* K.G. Medical College, Lucknow, India **The Ohio State University, Columbus, OH  &  Indian   Toxicology  Research   Centre,   Lucknow,   India