Dhanvantari Ayurveda Center Michael Dick, Ayurvedic Practitioner, Leesburg, Florida Call: 352-301-3183
The Ayurvedist®
Volume V Issue 3 July 2008
Health and Science in the News

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In This Issue Health in the News and More.... |
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1 |
Folk Medicine |
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2 |
General News |
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3 |
Scientific Studies |
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4 |
Dietary and Behavioral Interactions that May Pose Health Problems |
FOLK MEDICINE (People's Pharmacy)
Cinnamon extract may help control type 2 diabetes--shows improved insulin sensitivity and blood sugar control
Activia yogurt (from Dannon) eliminates flatus--probioitic Bifidus regularis helps digest milk sugar, lactose
Bananas relieve indigestion--a bite or two after meals relieves indigestion; powder also works
Castor oil to a bumped/damaged area seems to prevent bruising and hematomas
Toenail fungus?-- try hydrogen peroxide applied directly to infection
A new / different dental detoxification protocol: http://quantafoods.com/downloads/dental_detoxification_protocol.pdf
Norway, Sweden, Denmark ban mercury amalgam fillings. (Source: DAMS' Dental Truth, Feb 2008)
General News:
Since 1980's the total of medical schools using animal laboratories has fallen from over 100 to 10, with no school using dogs at all. WU, NYMC, St. Louis U, Duke, and Texas A&M are the latest to drop these practices. Source: Good Medicine Winter 2008
In the UK cancer studies are now carried out with human cancer tissue and body fluids. This is making for sounder conclusions in research by moving away from "animal models."
Students can now do virtual dissection on vertebrate anatomy by means of a digital program called Digital Frog II.
The Physician's Committee for Responsible Medicine (PCRM) has prepared a 900-page book: Nutrition Guide for Clinicians which it is distributing free of charge to all second-year medical students annually. It will be updated periodically to reflect changes in data and experience. It provides basic information on about 100 diseases and conditions, including risk factors and typical treatments, and also provides evidence-based information on how nutrition plays a role in prevention and treatment.
Scientific Studies:
Analysis of results from the massive Nurses Health Study I, II shows that those partaking in whole grain diets are less subject to type II diabetes. Source: PLos Med. 2007;4(8), 1385-1395
Atkins diet compared with Ornish diet and South Beach diet (n=18) (t=30 days), showed increased LDL, inflammation associated with blood clots, reduced blood vessel dilation suspected to cause long-term damage to blood vessels. The Ornish diet reduced LDL by over 25 pts while the South Beach diet lowered LDL only 10 pts. Source: Presentation in American Heart Assoc.'s Scientific Session, 11/6/07, Orlando, FL.
Western diet linked to increased birth defects, so concluded researchers in Netherlands (n=381). Diet is high in organ meat, red meat, processed meat, pizza, legumes, potatoes, French fries, condiments, and mayonnaise but low in fruits. Source: Obstet Gynecol 2007; 110: 378-384
The following abstracts have been chosen because they deal with the area of free-radical scavenging. Each article contributes something different to our understanding of this area--generally referred to as antioxidant activity. One aspect to note is that there are numerous tests for this function. Another aspect to note is that the body makes use of antioxidants in two different ways: enzymatic and non-enzymatic. One activates or works on processes involving enzymes and the other works with phytochemicals such as flavonoids, etc. Foods and food supplements that do these things are generally referred to as functional foods or functional medicines. Note also that cooking and other aspects bear on potency of effect.
Antioxidant Activity of Wheat Sprouts Extract In Vitro: Inhibition of DNA
Oxidative Damage
JFS: Food Chemistry and Toxicology
G. FALCIONI, D. FEDELI, L. TIANO, I. CALZUOLA, L. MANCINELLI, V. MARSILI,
AND G. GIANFRANCESCHI
ABSTRACT: Wheat sprouts contain a remarkable level of various antioxidants.
A fraction containing high amounts of powerful antioxidant glycoside
molecules has been isolated. In a dose- dependent manner, this fraction
reduces the lucigenin-amplified chemiluminescence produced by the superoxide
anion generated from the xanthine/xanthine oxidase system, thus indicating a
superoxide-scavenging activity. A protective effect of this wheat sprouts
fraction on the oxidative damage of pBR322 plasmid DNA induced by Fenton
reaction (Fe2+/H2O2) was subsequently demonstrated. Moreover, the results
reported here show that the amount of antioxidant compound strongly
increases during the germination phase, while scantly present in the wheat
germ, and virtually absent in the young wheat plant.
Keywords: wheat sprouts, antioxidants, chemiluminescence, DNA damage
Comparison of Antioxidant Potency of Commonly Consumed Polyphenol-Rich
Beverages in the United States
J. Agric. Food Chem. XXXX, xxx, 000 A
NAVINDRA P. SEERAM,† MICHAEL AVIRAM,§ YANJUN ZHANG,†
SUSANNE M. HENNING,† LYDIA FENG,† MARK DREHER,# AND DAVID HEBER*,†
Center for Human Nutrition, David Geffen School of Medicine, University of
California, Los Angeles,
California 90095; Lipid Research Laboratory, Technion Faculty of Medicine,
Rambam Medical
Center, Haifa, Israel; and POM Wonderful, LLC, Los Angeles, California 90064
A number of different beverage products claim to have antioxidant potency
due to their perceived high content of polyphenols. Basic and applied
research indicates that pomegranate juice (PJ), produced from the Wonderful
variety of Punica granatum fruits, has strong antioxidant activity and
related health benefits. Although consumers are familiar with the concept of
free radicals and antioxidants, they are often misled by claims of superior
antioxidant activity of different beverages, which are usually based only on
testing of a limited spectrum of antioxidant activities. There is no
available direct comparison of PJ’s antioxidant activity to those of other
widely available polyphenol¬rich beverage products using a comprehensive
variety of antioxidant tests. The present study applied (1) four tests of
antioxidant potency [Trolox equivalent antioxidant capacity (TEAC), total
oxygen radical absorbance capacity (ORAC), free radical scavenging capacity
by 2,2-diphenyl-1 -picrylhydrazyl (DPPH), and ferric reducing antioxidant
power (FRAP)]; (2) a test of antioxidant functionality, that is, inhibition
of low-density lipoprotein (LDL) oxidation by peroxides and malondialdehyde
methods; and (3) evaluation of the total polyphenol content [by gallic acid
equivalents (GAEs)] of polyphenol-rich beverages in the marketplace. The
beverages included several different brands as follows: apple juice (3),
açaí juice (3), black cherry juice (3), blueberry juice (3), cranberry juice
(3), Concord grape juice (3), orange juice (3), red wines (3), iced tea
beverages (10) [black tea (3), green tea (4), white tea (3)], and a major PJ
available in the U.S. market. An overall antioxidant potency composite index
was calculated by assigning each test equal weight. PJ had the greatest
antioxidant potency composite index among the beverages tested and was at
least 20% greater than any of the other beverages tested. Antioxidant
potency, ability to inhibit LDL oxidation, and total polyphenol content were
consistent in classifying the antioxidant capacity of the polyphenol-rich
beverages in the following order: PJ > red wine > Concord grape juice>
blueberry juice > black cherry juice, açaí juice, cranberry juice> orange
juice, iced tea beverages, apple juice. Although in vitro antioxidant
potency does not prove in vivo biological activity, there is also consistent
clinical evidence of antioxidant potency for the most potent beverages
including both PJ and red wine.
Effect of different cooking methods on the antioxidant activity of some
vegetables from Pakistan
International Journal of Food
Science and Technology 2008, 43, 560–567
Bushra Sultana,1 Farooq Anwar1* & Shahid Iqbal2
1 Department of Chemistry, University of Agriculture, Faisalabad 38040,
Pakistan
2 Department of Chemistry, University of Sargodha, Sargodha 40100, Pakistan
Summary The effects of different cooking methods (boiling, frying and
microwave cooking) on the antioxidant activity of some selected vegetables
(peas, carrot, spinach, cabbage, cauliflower, yellow turnip and white
turnip) were assessed by measuring the total phenolic contents (TPC),
reducing power and percentage inhibition in linoleic acid system. TPC
(gallic acid equivalents g/100 g of dry weight) and reducing power of the
methanolic extracts of raw-, microwaved-, boiled- and fried vegetables
ranged 0.333–2.97, 0.52–2.68, 0.48–2.08, 1.00–2.02 and 0.391–2.24,
0.822–1.10, 0.547–1.16, 0.910–4.07, respectively. The level of inhibition of
peroxidation ranged 71.4–89.0, 66.4–87.3, 73.2–89.2 and 77.4–91.3%,
respectively. Antioxidant activity of the vegetables examined was
appreciably affected because of varying cooking treatments. TPC of
vegetables, generally, decreased by boiling, frying and microwave cooking.
There was a significant (P < 0.05) increase in reducing power as a result of
frying. However, boiling and microwave cooking did not affect reducing
power. Inhibition of peroxidation increased by boiling and frying, whereas,
in contrast it was decreased by microwave cooking. The results of the
present investigation showed that all the cooking methods affected the
antioxidant properties of the vegetables; however, microwave treatment
exhibited more deleterious effects when compared with those of other
treatments. Thus an appropriate method might be sought for the processing of
such vegetables to retain their antioxidant components at maximum level.
Keywords: Antioxidant activity, cooking treatments, percentage inhibition of
peroxidation, reducing power, total phenolic contents, vegetables.
Preliminary
characterisation of peach cultivars for their antioxidant
capacity
International Journal of Food Science and Technology
2008, 43, 810–815
Silvia Tavarini,1 Elena Degl’Innocenti,1 Damiano Remorini,2
Rossano Massai2 & Lucia Guidi1* 1 Dipartimento di Chimica e
Biotecnologie Agrarie, University of Pisa, 56124 Pisa, Italy
2 Dipartimento di Coltivazione e Difesa delle Specie Legnose ‘G.
Scaramuzzi’, University of Pisa, 56124 Pisa, Italy
Summary: Eleven peach
cultivars (white- and yellow-flesh peaches, nectarines and
canning clingstone peaches) were assayed for their antioxidant
capacity and their content of some important organic compounds
as well as vitamin C, carotenoids and phenols. Antioxidant
capacity, determined by FRAP assay, varied between genotypes.
Those with the highest value of Ferric Reducing Antioxidant
Power (FRAP) were Federica and Springcrest. Antioxidant capacity
was correlated to the amount of organic components with
significant differences among the cultivars. In some case,
antioxidant capacity was related to phenol content, as in
yellow–flesh peaches. These results suggest the importance of
genotype for determining antioxidant capacity, which, in turn,
is related to the organic constituents, such as phenols, vitamin
C and carotenoids.
Keywords: Antioxidant capacity, ascorbic acid, carotenoids,
fruits, peach, phenols.
A review of the interaction among dietary antioxidants and reactive oxygen species
Journal of Nutritional Biochemistry
18 (2007) 567–579
Harold E. Seifrieda,4, Darrell E. Andersonb, Evan I. Fishera,
John A. Milnera aDivision of Cancer Prevention, National Cancer
Institute, Rockville, MD 20862, USA bThe Scientific Consulting
Group, Inc. Gaithersburg, MD 20878, USA
Abstract
During normal cellular activities, various processes inside of
cells produce reactive oxygen species (ROS). Some of the most
common ROS are hydrogen peroxide (H2O2), superoxide ion (O2 ),
and hydroxide radical (OH ). These compounds, when present in a
high enough concentration, can damage cellular proteins and
lipids or form DNA adducts that may promote carcinogenic
activity. The purpose of antioxidants in a physiological setting
is to prevent ROS concentrations from reaching a high-enough
level within a cell that damage may occur. Cellular antioxidants
may be enzymatic (catalase, glutathione peroxidase, superoxide
dismutase) or nonenzymatic (glutathione, thiols, some vitamins
and metals, or phytochemicals such as isoflavones, polyphenols,
and flavanoids). Reactive oxygen species are a potential
double-edged sword in disease prevention and promotion. Whereas
generation of ROS once was viewed as detrimental to the overall
health of the organism, advances in research have shown that ROS
play crucial roles in normal physiological processes including
response to growth factors, the immune response, and apoptotic
elimination of damaged cells. Notwithstanding these beneficial
functions, aberrant production or regulation of ROS activity has
been demonstrated to contribute to the development of some
prevalent diseases and conditions, including cancer and
cardiovascular disease (CVD). The topic of antioxidant usage and
ROS is currently receiving much attention because of studies
linking the use of some antioxidants with increased mortality in
primarily higher-risk populations and the lack of strong
efficacy data for protection against cancer and heart disease,
at least in populations with adequate baseline dietary
consumption. In normal physiological processes, antioxidants
effect signal transduction and regulation of proliferation and
the immune response. Reactive oxygen species have been linked to
cancer and CVD, and antioxidants have been considered promising
therapy for prevention and treatment of these diseases,
especially given the tantalizing links observed between diets
high in fruits and vegetables (and presumably antioxidants) and
decreased risks for cancer.
(C) 2007 Elsevier Inc. All rights reserved.
Keywords: Review; Reactive oxygen species; Antioxidants; Cancer;
Cardiovascular disease
A review of the
interaction among dietary antioxidants and reactive oxygen
species
Journal of Nutritional Biochemistry 18 (2007) 567–579
Harold E. Seifrieda,4, Darrell E. Andersonb, Evan I. Fishera,
John A. Milnera aDivision of Cancer Prevention, National Cancer
Institute, Rockville, MD 20862, USA bThe Scientific Consulting
Group, Inc. Gaithersburg, MD 20878, USA
Abstract
During normal cellular activities, various processes inside of
cells produce reactive oxygen species (ROS). Some of the most
common ROS are hydrogen peroxide (H2O2), superoxide ion (O2 ),
and hydroxide radical (OH ). These compounds, when present in a
high enough concentration, can damage cellular proteins and
lipids or form DNA adducts that may promote carcinogenic
activity. The purpose of antioxidants in a physiological setting
is to prevent ROS concentrations from reaching a high-enough
level within a cell that damage may occur. Cellular antioxidants
may be enzymatic (catalase, glutathione peroxidase, superoxide
dismutase) or nonenzymatic (glutathione, thiols, some vitamins
and metals, or phytochemicals such as isoflavones, polyphenols,
and flavanoids). Reactive oxygen species are a potential
double-edged sword in disease prevention and promotion. Whereas
generation of ROS once was viewed as detrimental to the overall
health of the organism, advances in research have shown that ROS
play crucial roles in normal physiological processes including
response to growth factors, the immune response, and apoptotic
elimination of damaged cells. Notwithstanding these beneficial
functions, aberrant production or regulation of ROS activity has
been demonstrated to contribute to the development of some
prevalent diseases and conditions, including cancer and
cardiovascular disease (CVD). The topic of antioxidant usage and
ROS is currently receiving much attention because of studies
linking the use of some antioxidants with increased mortality in
primarily higher-risk populations and the lack of strong
efficacy data for protection against cancer and heart disease,
at least in populations with adequate baseline dietary
consumption. In normal physiological processes, antioxidants
effect signal transduction and regulation of proliferation and
the immune response. Reactive oxygen species have been linked to
cancer and CVD, and antioxidants have been considered promising
therapy for prevention and treatment of these diseases,
especially given the tantalizing links observed between diets
high in fruits and vegetables (and presumably antioxidants) and
decreased risks for cancer.
(c) 2007 Elsevier Inc. All rights reserved.
Keywords: Review; Reactive oxygen species; Antioxidants; Cancer;
Cardiovascular disease
Effects of the ‘live
high–train low’ method on prooxidant/antioxidant balance on
elite athletes
European Journal of Clinical Nutrition (2008), 1–7&
2008 Nature Publishing Group All rights reserved 0954-3007/08
V Pialoux1, R Mounier1, E Rock2, A Mazur2, L Schmitt3, J-P
Richalet4, P Robach5, J Brugniaux4, J Coudert1 and N Fellmann1
1Laboratoire de Biologie des Activite´s Physiques et Sportives,
Faculte´ de Me´decine, Universite´ d’Auvergne, Clermont-Ferrand,
France; 2Equipe Stress Me´tabolique et Micronutriments, Unite´
de Nutrition Humaine UMR 1019, INRA, Saint Gene`s Champanelle,
France; 3Centre National de Ski Nordique, ID Jacobeys, Pre´manon,
France; 4Laboratoire Re´ponses cellulaires et fonctionnelles a`
l’hypoxie, Universite´ Paris 13, Bobigny, France and 5Ecole
Nationale de Ski et d’Alpinisme, Chamonix, France
Background/Objectives: We previously demonstrated that acute
exposure to hypoxia (3 h at 3000 m) increased oxidative stress
markers. Thus, by using the ‘living high–training low’ (LHTL)
method, we further hypothesized that intermittent hypoxia
associated with endurance training alters the prooxidant/antioxidant
balance. Subjects/Methods: Twelve elite athletes from the
Athletic French Federation were subjected to 18-day endurance
training. They were divided into two groups: one group (control
group) trained at 1200m and lived in hypoxia (2500–3000m
simulated altitude) and the second group trained and lived at
1200 m. The subjects performed an acute hypoxic test (10 min at
4800 m) before and immediately after the training. Plasma levels
of advanced oxidation protein products (AOPP), malondialdehydes
(MDA), ferric-reducing antioxidant power (FRAP), lipid-soluble
antioxidants normalized for triacylglycerols, and cholesterol
and retinol were measured before and after the 4800m tests.
Results: After the training, MDA and AOPP concentrations were
decreased in response to the 4800m test only for the control
group. Eighteen days of LHTL induced a significant decrease of
all antioxidant markers (FRAP, P¼0.01; a-tocopherol, P¼0.04;
b-carotene, P¼0.01 and lycopene, P¼0.02) for the runners. This
imbalance between antioxidant and prooxidant might result from
insufficient intakes in vitamins A and E. Conclusions: The LHTL
model characterized by the association of aerobic exercises and
intermittent resting hypoxia exposures decreased the antioxidant
status whereas the normoxic endurance training induced
preconditioning mechanisms in response to the 4800m test.
Keywords: intermittent hypoxia; endurance training; oxidized
lipids; AOPP; FRAP; a-tocopherol
A Comparative Study of Flavonoid Compounds, Vitamin C, and Antioxidant Properties of Baby Leaf
2330 J. Agric. Food Chem. 2008, 56,
2330–2340 Brassicaceae Species
ASCENSIÓN MARTÍNEZ-SÁNCHEZ, ANGEL GIL-IZQUIERDO, MARÍA I. GIL,
AND FEDERICO FERRERES*
Research Group on Quality, Safety and Bioactivity of Plant
Foods, Food Science and Technology Department, CEBAS-CSIC, P.O.
Box 164, Espinardo, Murcia E-30100, Spain
A comparative study of antioxidant compounds, flavonoids and
vitamin C, and also antioxidant activity was carried out in four
species of Brassicaceae vegetables used for salads: watercress
(Nasturtium officinale R. Br.), mizuna [Brassica rapa L. subsp.
nipposinica (L.H. Bailey) Haneltand], wild rocket [Diplotaxis
tenuifolia (L.) DC.], and salad rocket [Eruca vesicaria (L.)
Cav.]. The characterization of individual phenolic compounds by
HPLC-DAD-MS/MS-ESI in watercress and mizuna completes the
polyphenol study previously reported for wild rocket and salad
rocket. The qualitative study of flavonoids in watercress leaves
showed a characteristic glycosylation pattern with rhamnose at
the 7 position. Isorhamnetin 3,7-di-O-glucoside was identified
in mizuna leaves and may be considered a chemotaxonomical marker
in some B. rapa subspecies. Brassicaceae species showed
differences in the quantitative study of flavonoids, and the
highest content was detected in watercress leaves. Watercress
and wild rocket leaves had the highest content of vitamin C. The
antioxidant activity evaluated by different methods (ABTS, DPPH,
and FRAP assays) showed a high correlation level with the
content of polyphenols and vitamin C. In conclusion, the
Brassicaceae leaves studied, watercress, mizuna, wild rocket,
and salad rocket, presented a large variability in the
composition and content of antioxidant compounds. These baby
leaf species are good dietary sources of antioxidants with an
important variability of bioactive compounds.
KEYWORDS: Wild rocket; salad rocket; watercress; mizuna; phenolics; mass spectrometry; antioxidant activity
The induction of
human superoxide dismutase and catalase in vivo:
A fundamentally new
approach to antioxidant therapy
Sally K. Nelson a,b, Swapan K. Bose a, Gary K.
Grunwald c, Paul Myhill d, Joe M. McCord a,b,d,*
a
Webb-Waring Institute for Cancer, Aging and Antioxidant
Research, University of Colorado Denver Health Sciences Center,
Denver, CO 80262, USA
b
Department of Medicine, University of Colorado Denver Health
Sciences Center, Denver, CO 80262, USA
c
Department of Preventive Medicine and Biometrics, University
of Colorado Denver Health Sciences Center, Denver, CO 80262, USA
d
Lifeline Therapeutics, Denver, CO, USA
Abstract
A composition consisting of extracts of five widely studied
medicinal plants (Protandim) was administered to healthy human
subjects ranging in age from 20 to 78 years. Individual
ingredients were selected on the basis of published findings of
induction of superoxide dismutase (SOD) and/or catalase in
rodents in vivo, combined with evidence of decreasing lipid
peroxidation. Each ingredient was present at a dosage
sufficiently low to avoid any accompanying unwanted
pharmacological effects. Blood was analyzed before
supplementation and after 30 and 120 days of supplementation
(675 mg/day). Erythrocytes were assayed for SOD and catalase,
and plasma was assayed for lipid peroxidation products as
thiobarbituric acid-reacting substances (TBARS), as well as uric
acid, C-reactive protein, and cholesterol (total, LDL, and HDL).
Before supplementation, TBARS showed a strong age-dependent
increase. After 30 days of supplementation, TBARS declined by an
average of 40% ( p = 0.0001) and the age- ependent increase was
eliminated. By 120 days, erythrocyte SOD increased by 30% ( p <
0.01) and catalase by 54% ( p < 0.002). We conclude that modest
induction of the catalytic antioxidants SOD and catalase may be
a much more effective approach than supplementation with
antioxidants (such as vitamins C and E) that can, at best,
stoichiometrically scavenge a very small fraction of total
oxidant production.
(C) 2005 Elsevier Inc. All rights reserved.
Keywords: Superoxide dismutase; Catalase; Lipid peroxidation;
TBARS; Antioxidant; Protandim; Free radicals
Caraka lists 18 types of Incompatibility – Viruddhatva (See Sastry, pp. 344-349 for elaboration of these). Caraka lists many diseases that are the result of incompatibility (Ca.Su. 26.102), e.g.: impotency, blindness, herpes, ascites, erysipelas, schizophrenia, fistula-in-ano, syncope, stupor, flatulence, throat pain, anemia, autoimmunity, leucoderma, skin diseases, malabsorption syndromes, edema, inflammation, hyperacidity, fever, rhinitis, hereditary problems, and even death. The following are Caraka’s listing of 18 distinctive deleterious combinations:
It may be inferred from this listing that there exist many ways which food items can be harmful. When examining these more closely it will be found that many are energetic antagonisms (hot versus cold, etc.) while others will have their basis in chemistry (cooking peacock in castor oil). Some combinations are based upon exact proportions of similarity (honey and ghritam) and others of contrasting extremes to the body (dry, rough). Some combinations become problematic only because of the transformations they undergo during the digestive process (vipaka). Caraka discusses tastes and declares that a meal of a single taste would digest most easily (best). This statement is likely based upon direct experience and not upon some theoretical proposition. Ironically, Caraka goes on to declare that a meal of all 6 tastes is fundamentally the most prudent. A diet of meals having only a single taste would not be capable of supporting healthy physiology for long.
Bibliography
Shastry, J L N, Dravyaguna Vijñana, Chaukhambha, Varanasi, India, 2002, pp. 83-84
Sharma, PV. Translator, Caraka Samhita, Chowkhambha, Varanasi, India, 1992
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